Unmet Need: Effective promoter to express heterologous genes of interest in wheat.
Currently, expression of a heterologous gene of interest via transformation is achieved by using promoters from various sources including viruses (S35) or from other eukaryotes. Use of DNA from sources other than the target system requires the resulting transgenic plants to undergo additional regulatory processes. There is a need to find an effective wheat-based promoter.
The Technology: Use of wheat-based promoter.
Researchers at WSU have identified, cloned, and characterized a wheat-based promoter; a truncated version of which results in very high levels of expression of a target gene in all tissues and developmental stages. This particular truncated promoter sequence showed expression in all tissues of wheat with levels significantly higher than currently used promoters.
• Growth of transgenic wheat with favorable traits.
• The promoter shows expression in all tissues of wheat.
• Expression is significantly higher than currently used/available promoters.
• Resulting transgenic plants may not need to undergo additional regulatory processes.
Provisional patent application filed.